Competitive ELISA kit for the detection of anti-Rift Valley Fever (RVF) antibodies in serum or plasma from multiples species.
For research use only on human samples: see References (*).
- This test does not contain any virus particles
- Excellent analytical sensitivity, sensitivity and specificity, as proven in recent outbreaks
- Multi-species test: may be used on ruminants, horses, dogs and other species. For research use only on human samples: see References (*).
- Convenient and rapid format: ready-to-use, coated plates in strips, and all other components in liquid format. Results in less than 2 hours.
- Practical: you can easily check that all samples have been correctly deposited because the dilution buffer changes color upon addition of the sera
Specifications
| Method | Competitive ELISA |
| Species | Multiple species, including ruminants, camels, horses, dogs and others. For research use only on human samples: see References (*). |
| Specimens | Serum or plasma |
| Coated antigen | RVF Virus nucleoprotein (NP) |
| Conjugate | Anti-NP-HRP conjugate (concentrated 10X) |
Ordering Information
| Product code | RIFTC-4P | RIFTC-10P |
| Kit format | 4 plates | 10 plates |
| Reactions | 384 | 960 |
| Plate format | 12 x 8-well strips | 12 x 8-well strips |
REFERENCES
Comtet, L. et Pourquier, P. (IDvet), Marié, J-L. & Davoust, B. (French Defence Medical Service, Working group of animal epidemiology, Marseille, France), Cêtre-Sossah, C. (CIRAD, Montpellier, France). Preliminary validation of the ID Screen® Rift Valley Fever Competition Multi-species ELISA. Poster presented at the 2010 EAVLD meeting, Lelystad, The Netherlands.Ahmed B. et al. Unexpected Rift Valley Fever Outbreak, Northern Mauritania. Emerging Infectious Diseases. Vol. 17, No. 10. October 2011.Kortekaas, J. et al. European ring trial to evaluate ELISAs for the diagnosis of infection with Rift Valley fever virus. Journal of Virological Methods. September 2012.Antonis, A.F.G. et al. Vertical Transmission of Rift Valley Fever Virus Without Detectable Maternal Viremia. Vector-borne and zoonotic diseases. Vol. 13, No. X. May 2013.Bronsvoort B., Bagninbom J-M. et al.(2019). Comparison of Two Rift Valley Fever Serological Tests in Cameroonian Cattle Populations Using a Bayesian Latent Class Approach. Front. Vet. Sci. 6:258.(*) A.M. Mohamed, et al. Seroepidemiological survey on Rift Valley fever among small ruminants and their close human contacts in Makkah, Saudi Arabia, in 2011. Rev. sci. tech-Off. int. Epiz., 2014, 33 (3), 903-915(*) Opayele A,. Odaibo G. et al.(2018). Rift valley fever virus infection among livestock handlers inIbadan, Nigeria. Journal of Immunoassay and Immunochemistry, Vol. 39, No. 6, 609–621(*) Budodo R., Horumpende G. et al. (2020). Serological evidence of exposure to Rift Valley, Dengue and Chikungunya Viruses among agropastoral communities in Manyara and Morogoro regions in Tanzania: A community Survey. doi.org/10.1101/2020.01.16.908830(*) Ibrahim M., Schelling E.et al. (2020). Sero-prevalence of brucellosis, Q-fever and Rift Valley Fever in humans and livestock in Somali region, Ethiopia. doi: https://doi.org/10.1101/2020.01.31.928374More external references available upon request
ASSOCIATED PRODUCTS
Rift Valley Fever IgG-positive freeze-dried serum
| Product code | MRI-RIFT |
| Format | 1 ml vial (freeze-dried) |
| Description | Freeze-dried positive bovine serum to be used as internal reference material for quality control. |